Identification of a New Retrotransposable Element in Loblolly Pine

We initiated a project to locate the genomic position of fusiform rust resistance gene 1 (Fr1) in loblolly pine using fluorescent in situ hybridization (FISH). Four random amplified polymorphic DNA (RAPD) markers previously found to be tightly linked to Fr1 were cloned and sequenced, providing a total coverage of about 2 Kb. In order to obtain discernible signal of single-copy sequences using FISH, a minimum of 5 Kb of DNA is required. Therefore, GenomeWalker (Clontech) was used to obtain flanking genome clones and sequences for each of these markers.

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Author(s): M. N. Islam-Faridi, Alison M. Morse, Katherine E. Smith, John M. Davis, Saul A. Garcia

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 2005