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Enhancing The Productivity Of Hybrid Liriodendron Embryogenic Cultures

Characterized by fast growth and large, colored flowers, hybrids between yellow-poplar (Liriodendron tulipifera) and Chinese tuliptree (L. chinense) may be desirable for biomass production or ornamental use. Propagation of these hybrids via somatic embryogenesis using the suspension culture protocol previously developed for yellow-poplar has been unsuccessful. A high-frequency embryogenesis system for hybrid Liriodendron was established after testing several important culture variables, including 2,4-D concentration, sucrose, medium pH value, amino acids, casein hydrolysate (CH) and abscisic acid (ABA). Embryogenic cultures consisting of proembryogenic masses (PEMs) were initiated from immature hybrid seeds on yellow-poplar induction-maintenance (IM) medium.


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Author(s): Jianliang Dai, Scott A. Merkle

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 2001

Section: Posters