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U.S. Department of Agriculture USDA Forest Service Southern Regional Extension Forestry Southern Regional Extension Forestry

Factors regulating asexual embryogensis in vitro in longleaf pine

Embryogenic cultures of longleaf pine (Pinus palustris Mill.) were established from female gametophytes with intact zygotic embryos (at developmental stages 1 and 2) cultured on MSG and DCR basal media in darkness. Induction of embryogenic tissue was observed from explants cultured on both basal media containing glucose, or maltose or sucrose as carbon source. All four levels of each carbon source (15-90 g/L) used supported production of embryogenic tissue.


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Author(s): R. Nagmani, Alex M. Diner, G. C. Sharma

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1993

Section: Concurrent Session 3: Softwood Propagation