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U.S. Department of Agriculture USDA Forest Service Southern Regional Extension Forestry Southern Regional Extension Forestry

Developing codominant PCR markers in pines

Genetic markers which are easy to use and reveal abundant allelic variability provide powerful tools for many forest genetics applications. Because of their simplicity, methods using the polymerase chain reaction (PCR) are being developed for many plants. One class of PCR-based marker takes advantage of random amplification of polymorphic DNA (RAPD), but RAPD markers suffer several disadvantages related to their dominance and to their lack of comparability across pedigrees and between laboratories. To circumvent such problems, we are developing codominant PCR-based markers for pines. Our initial efforts have focussed on a subset of the cDNA clones previously used for constructing genetic maps. Nucleotide sequences from cDNA clones are used to design PCR primers for amplifying targeted fragments of genomic DNA from loblolly pine (Pinus taeda L.). Our pilot study demonstrates the feasibility of generating PCR markers in this way, but this method is complicated by the abundance of gene families in pine genomes.


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Author(s): D. E. Harry, David B. Neale

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1993

Section: Concurrent Session 5: Gene Mapping