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U.S. Department of Agriculture USDA Forest Service Southern Regional Extension Forestry Southern Regional Extension Forestry

Abscisic acid levels in embryos and megagametophytes of Pinus taeda L.

Abscisic acid (ABA) was quantified in developing zygotic embryos and megagametophytes of loblolly pine using indirect ELISA. On a per tissue basis, the amount of ABA present in the megagametophyte remained relatively constant at approximately 2.5 ng/megagametophyte. The ABA in embryos remained low during early to mid development, peaked to approximately 1.5 ng/ embryo, before declining and peaking a second time prior to cone ripening. On a g dry weight basis, ABA in megagametophytes exhibited a steady drop from approximately 1750 ng in early development to 127 ng at cone ripening. ABA in embryos was high during early development (2250 ng/g dry weight) and displayed two significant peaks middle to late development before dropping to approximately 600 ng/g dry weight at cone ripening. Peak levels of ABA in embryos (on a tissue basis) appear to occur shortly before the increase in dry weight accumulation.


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Author(s): R. H. Kapik, Ronald J. Dinus, Jeffrey F. D. Dean

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1993

Section: Concurrent Session 3: Softwood Propagation