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U.S. Department of Agriculture USDA Forest Service Southern Regional Extension Forestry Southern Regional Extension Forestry

Initiation of Adventitious Shoots from Vitreous Pinus palustris Micropropagules

Pinus palustris micropropagules of normal phenotype were generated from vitreous buds and shoots otherwise inappropriate for further use. Twenty-three vitreous shoots which had elongated to approximately 1 cm were cut to needles and stem segments, while younger buds in 25 collective vitreous clusters were pried/broken apart from each other. Tissues were then applied to a 0.8% agar-solidified Brown and Laurence mineral salts medium containing 44 uM 6-benzyladenine for 14 days.

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Author(s): Alex M. Diner

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1991

Section: Poster Abstracts