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U.S. Department of Agriculture USDA Forest Service Southern Regional Extension Forestry Southern Regional Extension Forestry

In Vitro Propagation of Pin Oak

Conditions were defined for in vitro propagation of pin oak (Quercus palustris Muenchh.). Competence of seedling explants averaged 93% on agar media compared to 41% in liquid culture; however, variable numbers (19-52%) of agar-produced shoots developed abnormally whereas normal shoot development occurred in liquid culture. Decreasing benzylaminopurine (BAP) from 8.9 to 0.089 µM in agar media did not affect competency but decreased the frequency of abnormal shoots and halved the yield of primary shoots. Shoots were subcultured in liquid modified Schenck and Hildebrandt or broadleaf tree media with 0.89 pM BAP. Eighty percent or more of the shoots could be rooted after indole-3-butyric acid treatment, but only plantlets rooted on filter paper bridges were successfully established in potting mix. Plantlets were acclimatized to greenhouse conditions. Keywords: Quercus palustris Muenchh., micropropagation, tissue culture.

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Author(s): R. T. Holland, P. Fenn, Feng H. Huang

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1989

Section: Concurrent Session 1A