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Factors Regulating Loblolly Pine (Pinus taeda L.) Somatic Embryo Development

The effect of medium components and culture conditions on somatic embryo development and the level of storage lipids (triglycerides) was studied in embryogenic callus of loblolly pine. A factorial experimental design was employed to test the effects of three factors on somatic embryo development: 1) basal medium, DCR (Gupta and Durzan, 1985) and MSG (Becwar et al., 1988); 2) culture condition, light versus dark; and 3) level of ABA. After 28 days on the treatments embryogenic calli were collected for somatic embryo counting and determination of triglyceride levels. Basal medium had a significant effect on the number of somatic embryos per gram of callus. Similarly, the level of ABA significantly affected somatic embryo development.

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Author(s): Michael R. Becwar, R. P. Feirer

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1989

Section: Concurrent Session 2A