Isozyme Identification of Competing Pollen Parents in Seed From Mixed Pollen Crosses Between Austrian Pines and Austrian X Japanese Red Pines

Pollen mixes were used to estimate pollen/ embryo competition and the effects of timeliness of competing pollens. Pollen parents of offspring were distinguished by electrophoretic banding patterns of alcohol dehydrogenase and glutamic-oxaloacetic transaminase. Pollen/embryo competition was estimated by applying 50:50 percent pollen mixes to several Austrian pine seed trees. Offspring from these mixes are produced in a 1:1 ratio in the absence of competition. The majority of crosses using mixed pollens of Austrian pine produced seedling offspring in the expected proportions. All Austrian and Japanese red pine pollen mixed produced offspring in proportions that deviated markedly from that expected. Native embryos were highly favored over hybrid embryos. The effect of timeliness was estimated by sequential pollen applications of the same conelet. The pollens of two different parents were applied separately one or two days apart. Both intra- and interspecific combinations showed that the first pollen received was substantially more effective than the second. Results are discussed in reference to pollen management and production of hybrids.

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Author(s): James J. Tobolski

Publication: Tree Improvement and Genetics - Northeastern Forest Tree Improvement Conference - 1982

Section: Session 4