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In Vitro Culture of American Elm Anthers

The objectives of this study were to examine the suitability of 14 nutritive media and five stages of microspore development for the culture of American elm anthers. Flower buds of branches from two trees were forced in the laboratory during February, March, and April. Anthers from these trees were plated on a total of 1262 petri dishes (five anthers per dish) to test the different media and the various development stages. Cultures were screened for production of aggregates of enlarged, multinucleate microspores. The frequency of formation of these aggregates ranged from 0 to 50 percent depending on the stage of anther development and on the particular medium. Attempts to induce cell division in the enlarged microspores have not yet been successful.

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Author(s): M. Keith Redenaugh, David F. Karnosky, Robert D. Westfall

Publication: Tree Improvement and Genetics - Northeastern Forest Tree Improvement Conference - 1977