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Identification of Phytophthora Diseases in Tennessee Nurseries

Mmbaga, M. T., Lamour, K., Donahoo, R., and Mrema, F. A. Phytopathology 97(7)Suppl:S78. 2007.

Phytophthora is a serious pathogen in field and container-grown trees in i Tennessee nurseries and is economically important in urban settings where it is associated with sporadic death of trees and shrubs. A survey for Phytophthora was done in mid-Tennessee where samples were collected from symptomatic and non-symptomatic plants. Direct isolation of Phytophthora from plant tissue including leaves, stem, and roots was done using a selective medium. Leaf-discs of Rhododendron and Pieris, Pine needles, and pear and apple fruits were used as baits to isolate Phytophthora spp. from soil and from irrigation water. All isolates were characterized morphological and representative of each morphological types were analysed using DNA sequence analysis of the ITS region.


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Author(s): M. T. Mmbaga, K. Lamour, R. S. Donahoo, F. A. Mrema

Section: Pest Management