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Home → Publications → American Chestnut Proceedings → 1992 → Isozyme Polymorphisms in Chinese Chestnut Cultivars

Isozyme Polymorphisms in Chinese Chestnut Cultivars

Chinese chestnut is one of the most genetically diverse species among Castanea spp. In China, more than 300 local cultivars have been recognized and divided into 6 distinct regional cultivar groups based on their original distribution and horticulturally important traits. However, less than 50 cultivars are commercially grown for nut production. The objectives of this study were to determine the level of isozyme polymorphisms in C. mollissima and evaluate the possibility of isozyme banding patterns for cultivar identification. Four enzyme systems (ACP, AAT, EST, PRX) were studied in 22 widely grown cultivars that represent four regional cultivar groups and 1 colonized population, using polyacrylamide gel isoelectric focusing of bud extracts. No clear zymograms of AAT were obtained in this study (data excluded). High level of isozyme polymorphisms of three enzyme systems were found on these 22 Chinese chestnut cultivars. There were 20, 15 and 13 phenotypic banding patterns observed for EST, PRX and ACP, respectively. Overall, 20 of 22 Chinese chestnut cultivars could be identified by one or more phenotypic banding patterns.


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Author(s): G. E. Boyhan, Hong Wen Huang, J. D. Norton, R. B. Abrahams

Publication: American Chestnut Proceedings - 1992