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Home → Publications → American Chestnut Proceedings → 1992 → Interactions Between Artificially Established Virulent Cryphonectria parasitica Cankers and Sources of Virulent and Hypovirulent Inoculum on American Chestnut Stems

Interactions Between Artificially Established Virulent Cryphonectria parasitica Cankers and Sources of Virulent and Hypovirulent Inoculum on American Chestnut Stems

The success of hypovirulent (hv) strains, as biological control agents for chestnut blight in forest settings, depends on their effective dissemination and interaction with virulent (v) strains. This study assessed the role of vegetative compatibility by examining the interactions between vegetatively compatible and incompatible v and hv strains of Cryphonectria parasitica artificially inoculated on American chestnut stems. Color mutants, a pcnb tolerant strain, and a morphologically distinct hv isolate were used so that interactions could be measured. Cankers were established on stems with a brown-pigmented strain of C. parasitica, allowed to develop for 8 wk, and then exposed for 10, 20 or 52 wk to vegetatively compatible or incompatible orangepigmented v or hv inoculum. Laboratory inoculated bark patches, placed against the bark 10 cm above the cankers, served as inoculum sources. Cankers were harvested and bark plugs removed and cultured. Resulting isolates were assessed for pigmentation, morphology and sensitivity to pcnb. Final canker dimensions also were evaluated.


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Author(s): C. R. Sypolt, D. L. Hobbins, Mark L. Double, William L. MacDonald

Publication: American Chestnut Proceedings - 1992