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Home → Publications → American Chestnut Proceedings → 1992 → Host Polyphenoloxidase Activity and Fungal Oxalate Production in the Host-Parasite Interaction Castanea sativa-Cryphonectria parasitica

Host Polyphenoloxidase Activity and Fungal Oxalate Production in the Host-Parasite Interaction Castanea sativa-Cryphonectria parasitica

Polyphenoloxidase (PPO) activity of European chestnut (Castanea sativa) bark tissues inoculated with the hypovirulent strain TR57/a of Cryphonectria parasitica and its monoconidial virulent isolate M18, was analyzed qualitatively and compared with activity in healthy control bark. PPO activity of C. sativa was higher during spring and fall season than during summer. An inverse relationship was found between PPO activity and size of the necrosis produced by the fungus. A selective inhibition of one isoenzyme by the virulent strain was found in isoelectrofocusing (IEF) experiments. Oxalate accumulation was found in necrotic chestnut bark tissues inoculated with the hypovirulent strain and with the virulent isolate. No significant differences were found in oxalate accumulation between the hypovirulent strain and the virulent isolate. No inhibition of PPO from healthy chestnut bark was found at different concentrations of oxalic acid.


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Author(s): Andrea Vannini, P. Magro, Isacco Beritognolo

Publication: American Chestnut Proceedings - 1992