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Home → Publications → American Chestnut Proceedings → 1992 → Enzyme Activity During Adventitious Rooting of Stoolbed Propageted Chestnut

Enzyme Activity During Adventitious Rooting of Stoolbed Propageted Chestnut

Chestnut propagation by the stoolbed technique, after girdling the lower portion of the shoots, has been satisfactorily employed for several years. In order to study the physiological mechanisms that stimulate rooting under these conditions, with special reference to the activity of some enzymes, 10-yr-old stoolbeds of the cultivar `Marigoule' (Castanea crenata x C. sativa) were subjected to: 1) girdling and etiolation; 2) girdling; 3) etiolation; and, 4) control, or totally untreated stoolbeds. Treatments were made on 20 June, 2 mo after bud break. Shoot samples (basal and sub-apical shoot portions) were collected during the period between 23 May and 30 November, 1 mo after leaf fall, for the determination of the activity of the following enzymes: IAA-oxidase, peroxidase, polyphenoloxidase.


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Author(s): A. Masia, Andrea Fabbri, Francesco Ferrini, P. L. Pisani

Publication: American Chestnut Proceedings - 1992