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Home → Publications → American Chestnut Proceedings → 1982 → Propagation of American Chestnut In Vitro

Propagation of American Chestnut In Vitro

ABSTRACT.--Axillary shoots developed on mature embryos of American chestnut seeds which were cultured on 6-benzylaminopurine (BAP-supplemented Murashige and Skoog (MS) medium. These shoots could be multiplied by subculture on MS medium with reduced BAP concentration. Rooting of some of these shoots occurred when they were placed on 3-indolebutyric acid (IBA)-supplemented medium followed either by culture on IBA - free medium, or by planting invermiculite and placing them in a greenhouse mist propagation bed. Plantlets have been successfully transferred to soil in pots and grown outdoors. Axillary shoots have also been obtained from buds of 4-month-old greenhouse-grown seedlings.


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Author(s): Roy N. Keys, Franklin C. Cech

Publication: American Chestnut Proceedings - 1982