Mycelium Production in Liquid Media By Several Normal, Hypovirulent and Converted Endothia Parasitica Isolates

ABSTRACT.--Tests were conducted with several liquid media to determine if mycelium production could be used to differentiate between normal, hypovirulent, and in vitro converted isolates of Endothia parasitica. Difco potato dextrose broth generally supported more mycelium formation than any other medium tested. Arginine supported the production of more mycelium than any other amino acid tested and more than the inorganic nitrogen source, ammonium tartrate. Cellobiose, glucose and fructose supported more mycelium formation in a defined medium with arginine as the nitrogen source than other carbon sources tested. Usually those hypovirulent isolates that grow faster and are white or slightly pigmented on Difco potato dextrose agar produced more mycelium than those hypovirulent isolates that grow slower and pigment more intensely. Mycelium production varied among normal isolates in each medium, and that produced by converted isolates often differed markedly from that produced by either of the original isolates. Mycelium production by four isolates with identical genetic backgrounds but with varying dsRNA components varied in some defined media. The normal isolates often produced more mycelium than the hypovirulent isolates, but mycelium production did not differentiate among the hypovirulent ones. Although mycelium production varied among the test media and among isolates, this cultural characteristic could not be used to reliably differentiate between the normal and hypovirulent isolates tested. Mycelium production on certain media may have been used for quantitatively differentiating those hypovirulent isolates that grow more rapidly and produce slight pigment from those that grow more slowly and pigment more intensely.

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Author(s): Dale F. Hindal, T. G. Hagen

Publication: American Chestnut Proceedings - 1982