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Home → Publications → American Chestnut Proceedings → 1981 → Double-strand RNA from Protoplasts of Endothia parasitica (EP-49)

Double-strand RNA from Protoplasts of Endothia parasitica (EP-49)

Protoplasts were obtained from 3- to 4- day-old mycelium of EP-49, a European hypovirulent strain of Endothia parasitica, by glucuronidase-cellulose digestion of the cell walls in an isotonic solution. Double-stranded RNA (dsRNA) extracted from osmotically lysed protoplasts was compared with dsRNA from glass-bead-homogenized mycelium of EP-49 using polyacrylamide gel electrophoresis. The two dsRNA preparations exhibited the same gel pattern. These data indicate that glass-bead homogenization does not result ire fragmentation of the dsRNA genome.


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Author(s): Kevin Gillies, Neal K. Van Alfen, John Dimmone, James T. Bowman

Publication: American Chestnut Proceedings - 1981