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Home → Publications → Climate Change / Assisted Migration → Application of random amplified polymorphic DNA (RAPD) to detect genetic variation in Norway spruce

Application of random amplified polymorphic DNA (RAPD) to detect genetic variation in Norway spruce

Heinze, B., Westcott, R., Schmidt, J., Glossl, J. 1996. New Forests, Volume 11: 173-184
Journal Article
Development

Europe

The RAPD assay is a screening method for genetic variation; in Norway spruce biology research, it may find many applications . We have investigated into its suitability for Norway spruce genetics by optimizing the protocol, testing for stability in clones, especially tissue culture clones, assessing the variation in a seed sample, and checking for correct Mendelian segregation in haploid megagametophytes. The RAPD assay produced numerous genetic markers quickly. The data obtained gave insight into the genetic make-up of clones and seed.