Home Publications Tree Improvement and Genetics Southern Forest Tree Improvement Conference 31st Southern Forest Tree Improvement Conference (2011) Influence of American versus Chinese chestnut genome proportion on somatic embryogenesis induction

Influence of American versus Chinese chestnut genome proportion on somatic embryogenesis induction

Somatic embryogenesis (SE) has been successfully applied for clonal propagation of American chestnut trees, including transgenic trees engineered with genes potentially conferring resistance to chestnut blight. It may also be useful for clonally propagating blight-resistant trees produced by a hybrid backcross breeding program, in which American trees are hybridized with blightresistant Chinese chestnut trees, followed by backcrossing to American trees. However, to date there have been no reports of SE in either Chinese chestnut or hybrid backcross material. We tested the effects that Chinese chestnut genome proportion and pollination type (control versus open pollination) have on the success of SE induction using a standard protocol for culturing American chestnut. None of the material that had been control-pollinated produced embryogenic cultures.


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Author(s): Christine T. Holtz

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