Expression and Stability of Transgenes in Aspen-Populus

We have employed Populus as a model system to investigate questions regarding stability and expression of foreign genes in transgenic trees. Several clones of European aspen (P. tremula) and hybrid aspen (P. tremula x P. tremuloides) were genetically transformed, using Agrobacterium as a vector system, with different gene constructs, in particular rolC. In order to test the expressive control of promoters, we have employed two types promoters: 35S from cauliflower mosaic virus, and light-inducible rbcS from potato, for control of roIC gene expression in transgenic aspens.


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Author(s): M. R. Ahuja, M. Fladung

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1997

Section: Contributed Presentations: Full Papers

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