Chromosome Structure and Molecular Cytogenetics of Southern Pines

Molecular cytogenetics is the application of the techniques of molecular biology to the study of chromosome structure and function. Molecular cytogenetics principally involves the application of fluorescent in situ hybridization or FISH, a technique that allows the localization of specific sequences on metaphase chromosomes and interphase nuclei. Other techniques, such as primed in situ amplification, in situ polymerase chain reaction, and total genomic in situ hybridization are also important. These techniques permit the correlation of genetic linkage groups and physical chromosomes. The techniques also allow the localization of repetitive sequences, and the localization of genes in gene families which often have greatly different copy numbers at each locus and intra locus polymorphism, making them difficult to map using other mapping techniques.


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Author(s): M. E. Mason, R. L. Doudrick

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1997

Section: Contributed Presentations: Extended Abstracts

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