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Effect of Humidity during Artificial Extraction on the subsequent Vigor of Pine Pollen

My objective was to determine how different relative humidities during artificial extraction affect pollen vigor. The length of tubes produced by pollen grains germinating in distilled water was used as a judge of vigor. Pollen came from four jack pines and four eastern white pines. Catkins were collected from the trees just after the formation of the generative and tube cells of individual pollen grains. This was during the fourth week of April for jack pine and the third week of May for white pine. The catkins were kept in chambers maintained at relative humidities of 15 to 20, 30 to 40, 60 to 70, and 100 percent until the pollen was released.


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Author(s): Russell A. Ryker

Publication: Tree Improvement and Genetics - Central States Forest Tree Improvement Conference - 1962