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The Forest Service National Center for Reforestation, Nurseries, and Genetic Resources (RNGR) is a leading source of technical information for nurseries and land managers regarding production and planting of trees and other native plants for reforestation, restoration, and conservation.

 
NPN Protocol Details Image

Dicranopteris (linearis)

Leif Erickson
Botanist
3860 Mānoa Road
Honolulu, Hawaii 96822
(808) 988-0456
mickle@hawaii.edu

Family Scientific Name: Gleicheniaceae
Family Common Name: Forked Ferns
Scientific Name: Dicranopteris linearis (Burm.f.) Underw.
Common Synonym: Dicranopteris dichotoma (Thunb.) Bernh.
Common Name: Bengkawang (Malay), Coral fern, Dilim, False staghorn fern, Uluhe (Hawaiian), Hasam (Lampung), Kiku kachoei (Thai), Kilob (Tagalog), Kloutervaring, Kut pit (Thai), Kut taem (Thai), Máng ch'í (Chinese), Paku andam (Sudanese), Old world forked fern
Species Code: DILI
Ecotype: Puʻu Pia trail, Honolulu, Hawaiʻi, U.S.A.
General Distribution: Mesic to wet forests. Hawaiʻi, all major islands. Tropical Africa, Asia, Australia, New Zealand, Polynesia, and Indonesia.
Propagation Goal: Plants
Propagation Method: Seed
ProductType: Container (plug)
Stock Type: Spore
Time To Grow: 5 months
Target Specifications: Sporophyte development of immature fronds. Hight: 5cm
Propagule Collection: Mature fertile fronds were collected when sporangia begin to change from green to yellow. Spores likely dispersed if sporangia are brown and frond is beginning to senesce.
Propagule Processing: Collected fertile fronds were washed for removal of foreign spores and left to dry for 1-2 hours. The fronds were then placed into folded paper packets for one week, ensuring the dehiscence of the sporangia (Romanchak, et al., 2005). After spore dissemination, the packets were opened and the loose spores were transferred to a 53 μm sieve (Cole-Parmer®, No. 270) in order to remove any debris.
Pre-Planting Treatments: Individual 5.0 mg packets of D. linearis spores were portioned using an analytical balance (Mettler AE100). A fine paintbrush was used to disperse spores onto the medium. The brush was held approximately 5 cm above the medium’s surface and spores were sown by lightly tapping the brush.
Growing Area Preparation/
Annual Practices for Perennial Crops:
Sphagnum moss (Spagmoss, New Zealand) was dampened and microwaved for 5 minutes for sterilization. The moss was then placed in clear plastic container (15x7.5x2.5cm). closed and allowed time to cool. Containers were placed in an air-conditioned room (20°C), on metal shelving, covered in plastic sheeting, under cool white fluorescent lights (~50 μmol⁻²s⁻¹ ) (14/24 hr). Medium should be wetted with deionized water once a month or if no condensation is visible on the container. Gametophytes were sprayed with fertilizer (Organics Rx, Sea-kelp 100 (1tbs/gal)) 30-40 days after sowing.
Establishment Phase: Spores to gametophyte.
Length of Establishment Phase: 1 month
Active Growth Phase: Gametophytes to sporophyte.
Length of Active Growth Phase: 5 months
Length of Storage: Spores can be stored in fridge. Loss of spore viability after 1 year
Other Comments: This protocol was authored by Leif Erickson, Samuel Champine-tocher, and Nellie Sugii. University of Hawaiʻi: Mānoa, Harold Lyon Arboretum: Hawaiian Rare Plant Program, 3860 Mānoa Road, Honolulu, H.I. 96822, USA. Email - merickson@atlantabg.org; champine@hawaii.edu; sugii@hawaii.edu
References: Romanchak, E., R.A. Criley and N. Sugii. 2005. The propagation of Uluhe fern (Dicranopteris Linearis): vegetative versus spores. International Plant Propagators Society, Combined Proceedings. 55:517-519.; Palmer, Daniel D. Hawaiʻi's Ferns and Fern Allies. Univ of Hawaii Pr, 2008.

Citation:

2019. Propagation protocol for production of Container (plug) Dicranopteris linearis (Burm.f.) Underw. Plants Spore; Honolulu, Hawaii. In: Native Plant Network. URL: https://NativePlantNetwork.org (accessed 2024/07/02). US Department of Agriculture, Forest Service, National Center for Reforestation, Nurseries, and Genetic Resources.