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The Forest Service National Center for Reforestation, Nurseries, and Genetic Resources (RNGR) is a leading source of technical information for nurseries and land managers regarding production and planting of trees and other native plants for reforestation, restoration, and conservation.

 
NPN Protocol Details Image

Hackelia (venusta)

Anthony S. Davis
Director and Assistant Professor
University of Idaho - Center for Forest Nursery and Seedling Research
University of Idaho, PO Box 441137
Moscow, Idaho 83844-1137
208.885.3888
208.885.6226 (fax)
asdavis@uidaho.edu
http://seedlings.uidaho.com/

Family Scientific Name: Boraginaceae
Family Common Name: Borage family
Scientific Name: Hackelia venusta (Piper) St. John
Common Name: Showy stickseed
Species Code: HAVE
Ecotype: Dry, eastern slopes of Cascade Mtn range with ponderosa pine.
General Distribution: This species of Hackelia is only found in Chelan County in the Washington Cascade Mountain Range.
Propagation Goal: plants
Propagation Method: vegetative
ProductType: Container (plug)
Stock Type: 165 ml capacity Hillson Rootrainers
Time To Grow: 16 Weeks
Target Specifications: 15-cm-long, firm root plug
Propagule Collection: Propagules were rooted microshoots.
Pre-Planting Treatments: Rooted propagules were removed from sterile, glass tubes. The roots were gently washed under running water to remove any remaining agar.
Establishment Phase: Rooted propagules were planted in rootrainers containing a 1:1 volume of Sunshine peat and perlite. Relative humidity was 90% and progressively decreased to ambient over the course of 4 weeks. Plantlets were kept in 70% shade and slowly acclimated to normal greenhouse sunlight over 4 weeks.
Length of Establishment Phase: 1 month
Active Growth Phase: Acclimated plantlets were then fertilized with 20:20:20 (N:P2O5:K2O) at 200 ppm N twice a week for 8 weeks.
Length of Active Growth Phase: 2 months
References: Edson, JL., Leege-Brusven, A., Everett, RL., and Wenny, DL. 1996. Minimizing growth regulators in shoot culture of an endangered plant, Hackelia venusta (Boraginaceae). In Vitro Cell. Dev. Biol. - Plants 32:267-271.

Citation:

Brusven, Annette D.L.; Edson, John L.; Everett, Richard L.; Wenny, David L. 2001. Propagation protocol for production of Container (plug) Hackelia venusta (Piper) St. John plants 165 ml capacity Hillson Rootrainers; University of Idaho - Center for Forest Nursery and Seedling Research Moscow, Idaho. In: Native Plant Network. URL: https://NativePlantNetwork.org (accessed 2024/07/03). US Department of Agriculture, Forest Service, National Center for Reforestation, Nurseries, and Genetic Resources.