Hackelia (venusta)
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Anthony S. Davis Director and Assistant Professor University of Idaho - Center for Forest Nursery and Seedling Research University of Idaho, PO Box 441137 Moscow, Idaho 83844-1137 208.885.3888 208.885.6226 (fax) asdavis@uidaho.edu http://seedlings.uidaho.com/ |
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| Family Scientific Name: | Boraginaceae | ||
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| Family Common Name: | Borage family | ||
| Scientific Name: | Hackelia venusta (Piper) St. John | ||
| Common Name: | Showy stickseed | ||
| Species Code: | HAVE | ||
| Ecotype: | Dry, eastern slopes of Cascade Mtn range with ponderosa pine. | ||
| General Distribution: | This species of Hackelia is only found in Chelan County in the Washington Cascade Mountain Range. | ||
| Propagation Goal: | other propagules | ||
| Propagation Method: | vegetative | ||
| ProductType: | Propagules (seeds, cuttings, poles, etc.) | ||
| Stock Type: | micropropagated plantlets | ||
| Time To Grow: | 12 Weeks | ||
| Target Specifications: | Our goal was to multiply explants and obtain microshoots that were at least 2 cm in height and ready to be rooted. | ||
| Propagule Collection: | We excised 1.5 to 2.5 cm long shoot tips from newly emerging plants just after the snow melted in the Cascade Range. Shoot tips were refrigerated and transported to the micropropagation lab in Moscow, Idaho. | ||
| Pre-Planting Treatments: | Upon reaching the lab, shoot tips were immediately defoliated and surface sterilized for 20 min in a 1% solution of NaClO. They were then rinsed3 times in sterile distilled water. | ||
| Growing Area Preparation/ Annual Practices for Perennial Crops: |
Shoot tips (explants) were placed on Murashige and Skoog (MS) medium in glass test tubes covered with cotton plugs. Explants were incubated on shelves under cool-white fluorescent light with an 18h:6h (light:dark) photoperiod. Temperatures were kept at 22 degrees C (night) and 27 degrees C (day). | ||
| Establishment Phase: | Explants were grown on the MS medium for 1 month. This gave them time to acclimate to a new growing environment and begin elongating. | ||
| Length of Establishment Phase: | 1 month | ||
| Active Growth Phase: | After the 1 month establishment phase, explants were removed from test tubes, and any new shoots were excised and placed on fresh MS medium containing 0.04 micromolar benzyl adenine (BA). This procedure was repeated every 4 weeks for 2 months until we had the desired number of shoots. | ||
| Length of Active Growth Phase: | 2 months | ||
| Hardening Phase: | Shoots were excised from the cultures and transferred to glass tubes containing MS medium with 2 micromolar of indole acetic acid (IAA) added. Rooted shoots were then ready to be acclimated to greenhouse conditions. | ||
| Length of Hardening Phase: | 1 month | ||
| Harvesting, Storage and Shipping: | If necessary, shoots can be stored in a refrigerater until ready for rooting. We found that they could be stored up to 5 months in a dark cooler with minimal damage. It is best, though, if shoots can go directly into the next phase, whether it be additional multiplication or rooting. | ||
| References: |
Edson, J., Leege-Brusven, A., Everett, R., and Wenny, DL. Minimizing growth regulators in shoot culture of an endangered plant, Hackelia venusta (Boraginaceae). In Vitro Cell. Dev. Biol. -Plant 32:267-271; 1996. Murashige, T. and Skoog, FL. A revised medium for raped growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497; 1962. |
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Citation:
Brusven, Annette D.L.; Edson, John L.; Everett, Richard L.; Wenny, David L. 2001. Propagation protocol for production of Propagules (seeds, cuttings, poles, etc.) Hackelia venusta (Piper) St. John other propagules micropropagated plantlets; University of Idaho - Center for Forest Nursery and Seedling Research Moscow, Idaho. In: Native Plant Network. URL: https://NativePlantNetwork.org (accessed 2024/07/03). US Department of Agriculture, Forest Service, National Center for Reforestation, Nurseries, and Genetic Resources.
