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Advancing toward a transcriptional map of the loblolly pine genome

We are evaluating several approaches to identify and map expressed genes with known functions to create a transcriptional, or gene, map of the loblolly pine (Pinus taeda L.) genome. Several approaches to identify expressed genes are: 1) sequencing random complementary DNA (cDNA) from loblolly pine, 2) obtaining gene probes from other species of Pinaceae, 3) cloning gene fragments using the polymerase chain reaction, and 4) constructing cDNA libraries that represent specific classes of expressed genes. cDNA clones representing expressed genes are used as probes to detect and map RFLP loci. The putative identities of nine mapped cDNA's for loblolly pine have been determined, eight RFLP loci have been mapped using probes from P. sylvestris, and a segment of a phytochrome gene has been cloned using PCR primers designed from Arabidopsis thaliana phytochrome coding sequences. The results of our pilot studies will help determine the future emphases of our gene mapping efforts.


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Author(s): S. M. Colby, A. T. Groover, C. S. Kinlaw, D. E. Harry, David B. Neale

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1993

Section: Concurrent Session 4: Breeding and Progeny Testing