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Home → Publications → Tree Improvement and Genetics → Southern Forest Tree Improvement Conference → 17th Southern Forest Tree Improvement Conference (1983) → Adventitious Shoot Formation On Embryos Of Pinus Palustris Mill., P. Elliottii Engelm. And P. Palustris X Elliottii.

Adventitious Shoot Formation On Embryos Of Pinus Palustris Mill., P. Elliottii Engelm. And P. Palustris X Elliottii.

Adventitious shoot formation on the embryos of longleaf pine (P. palustris Mill.), slash pine (P. elliottii Engelm.) and their hybrid (P. palustris x elliottii) were compared. Using modified procedures of Reilly and Brown (1976), entire mature embryos were placed in a liquid media of Blaydes major salts, iron as Fe-EDTA, minor salts of Brown (Sommer and Brown, 1980), Doy's vitamins and 2% sucrose. The media was supplemented with napthaleneacetic acid at concentrations of 0.1, 0.01 and 0.001 mg./1. and 6-benzylaminopurine at concentrations of 1, 5, 10, 15 and 20 mg./1. The experimental design was a 3x5 factorial, with 20 explants of each species established for each hormone combination. The embryos were kept in the liquid media for ten days and then were transferred to a solid media of the following composition: major salts of Risser and White at half-strength, iron as Fe-EDTA, minor salts of Brown, Doy's vitamins, 1% sucrose and 0.6% Phytagar.


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Author(s): Michael Stine, Harry E. Sommer

Publication: Tree Improvement and Genetics - Southern Forest Tree Improvement Conference - 1983