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Increased growth of hybrid spruce bud cultures on stationary liquid medium

The effects of using liquid medium to increase growth of bud cultures of Spartan spruce (Picea glauca X P. pungens) were examined. The goals of the study were to improve the survival and elongation frequencies of the cultured buds, and to develop a culture system that could be used to initiate cultures from mature spruce trees during any season of the year. All cultures were initiated with buds excised from lateral branches of 10 year-old Spartan spruce. Bud scales were removed under a disecting microscope, and the upper two-thirds of the buds were cultured on both agar-solidified and liquid medium containing 10-4 M IAA and 10-6 M kinetin. No surface sterilization techniques were used. After four weeks all cultures were evaluated for average weight, needle elongation frequency, lateral shoot development, shoot elongation, and callus induction frequency. Cultures on liquid medium exceeded those on agar-solidified medium for all traits. Basal callus formation has occurred on these shoots, but no actual roots have been observed. Additional keywords: Tissue culture, mature spruce, shoot growth.


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Author(s): Daniel E. Keathley

Publication: Tree Improvement and Genetics - North Central Tree Improvement Conference - 1983