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In vitro axillary shoot formation and rooting in black walnut mature embryos

Mature embryos of black walnut were grown on a nutrient medium containing benzylaminopurine (BAP) at 2 mg/1, indoleacetylphenylalanine (IAAPhe) at 2 mg/1, or these two growth regulators in combination, each at 2 mg/l. The development of axillary shoots was maximal when both growth regulators were used. BAP alone gave some axillary bud release while IAAPhe alone produced a long slender stem axis which was devoid of axillary shoots. Excised axillary shoots formed adventitious roots readily when cultured on one-half strength MS basal medium containing indolebutyric acid at 1 mg/l. Additional keywords; Embryo culture, axillary bud development, Juglans nigra.


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Author(s): John L. Caruso

Publication: Tree Improvement and Genetics - North Central Tree Improvement Conference - 1983